@article{oai:dwcla.repo.nii.ac.jp:00002383,
 author = {羽森, 真美 and 清水, 恭子 and 佐藤, 綾花 and 芝田, 信人 and 杉浦, 幸雄 and 根木, 滋 and HAMORI, Mami and SHIMIZU, Kyoko and SATO, Ayaka and SHIBATA, Nobuhito and SUGIURA, Yukio and NEGI, Shigeru},
 journal = {総合文化研究所紀要, Bulletin of Institute for Interdisciplinary Studies of Culture Doshisha Women’s College of Liberal Arts},
 month = {Jul},
 note = {application/pdf, AN10052143-20220729-141, Sortase A is a transpetidase from Staphylococcus aureus that recognizes five specific amino acid sequences, LPETG, and binds them to polyglycine on the cell wall surface, immobilizing the target protein on the cell wall surface. Sortase A contains one Ca2＋ as a co-factor in its structure, and Ca2＋ plays an important role in the enzymatic reaction. First, the time course of the enzymatic reaction, the optimal Ca2＋ concentration, and the optimal reaction temperature were investigated using wild-type Sortase A. Next, we focused on E105, an amino acid residue that has been reported to play an important role in Ca2＋ binding. We prepared point mutants in which E105 was replaced with non-coordinating Ala residues and coordinating amino acid residues (Asp, Cys, His) with Lewis base moieties on the side chains and examined the enzymatic reaction in the presence of Ca2＋ and its cognate elements, Mg2＋ and Sr2＋.
The result was the clarification of the optimal Ca2＋ concentration and the optimal reaction temperature range for the enzymatic reaction. In the case of Ca2＋ in the wild-type and E105 mutants, the reactivity was WT ≈ E105D＜E105C＜E105A＜E105H, indicating that the enzymatic reaction is affected by the Lewis basicity of the ligand and the steric structure of the side chain. For Sr2＋, the same trend of reactivity as Ca2＋ was observed, but the overall reactivity decreased. However, for Mg2＋, reactivity was significantly decreased in all cases, indicating the significant effect of ionic radius on the enzymatic reaction., 論文},
 pages = {141--150},
 title = {ペプチド転移酵素Sortase A が触媒するtranspeptidation 反応に対するCa2＋濃度、反応温度、E105点変異、外因性金属イオンの効果に関する研究},
 volume = {39},
 year = {2022},
 yomi = {ハモリ, マミ and シミズ, キョウコ and サトウ, アヤカ and シバタ, ノブヒト and スギウラ, ユキオ and ネギ, シゲル}
}