{"created":"2023-05-15T14:06:38.133628+00:00","id":1887,"links":{},"metadata":{"_buckets":{"deposit":"06173e75-0cd8-4cae-b6fc-1405cbd00b53"},"_deposit":{"created_by":19,"id":"1887","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"1887"},"status":"published"},"_oai":{"id":"oai:dwcla.repo.nii.ac.jp:00001887","sets":["185:345","20:21"]},"author_link":["5960","6346","6347","5964"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-01-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"19","bibliographicPageStart":"13","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"同志社女子大學學術研究年報"},{"bibliographic_title":"Doshisha Women's College of Liberal Arts annual reports of studies","bibliographic_titleLang":"en"}]}]},"item_10002_description_19":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10002_description_35":{"attribute_name":"ID","attribute_value_mlt":[{"subitem_description":"AN0016561X-20200818-13","subitem_description_type":"Other"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"白血球の一種マクロファージ（Mφ）は、自然免疫と獲得免疫の両方に関与する免疫細胞である。さらに、Mφは、種々の生理活性物質を産生し、多くの炎症性疾患を始めとする様々な疾患の病態形成に深く関わっている。Mφには、ムスカリン性およびニコチン性アセチルコリン受容体 (mAChRおよびnAChR) が発現している。Mφ上のα7型 nAChRは、炎症性サイトカイン腫瘍壊死因子-αの遊離抑制や抗原提示機能の調節に関与していることが報告されている。本研究では、MφにおけるAChRの役割を明らかにしていく目的で、Mφの活性化がAChRの遺伝子発現に及ぼす影響を検討した。さらに、Mφの炎症性遺伝子発現制御機構におけるAChRの生理的役割を検討した。ヒトマクロファージ様細胞株U937細胞をMφモデルとして使用した。U937細胞において、LPSによる活性化は、M1、M3、M5サブタイプmAChR mRNA、およびα4、β2 サブユニットnAChR mRNAの発現を増大させた。U937細胞において、LPSは、COX-2 mRNAの発現を増大させた。しかしながら、mAChRあるいはnAChRの活性化だけでは、COX-2 mRNA発現は影響を受けなかった。nAChRの活性化は、LPSによるCOX-2 mRNA発現の増大を抑制したが、mAChRの活性化はLPSによるCOX-2 mRNA発現の増大には影響しなかった。以上の結果より、Mφの活性化によりmAChRおよびnAChRの発現が増大することが明らかとなった。さらに、U937細胞におけるCOX-2の発現制御機構において、TLR4を介したMφの活性化によるCOX-2 mRNAの発現増大は、nAChRを介した機構によって抑制されることが示唆された。\n\nA type of white blood cells, macrophage (Mφ) is an immune cell involved in both innate and acquired immunity. Furthermore, Mφ produces various physiologically active substances and is deeply involvedin the pathogenesis of various diseases including many inflammatory diseases. Mφs express muscarinic and nicotinic acetylcholine receptors (mAChR and nAChR). It has been reported that α7 type nAChR on Mφ is involved in reducing the release of pro-inflammatory cytokine tumor necrosis factor-α and regulating antigen presentation function. In this study, we investigated the effect of Mφ activation on AChR gene expression in order to clarify the role of AChR in Mφ. Furthermore, we investigated the physiological role of AChR in the regulation mechanism of inflammatory gene expression of Mφ. Human macrophage-like cell line U937 cells were used as an Mφ model. In U937 cells, the presence of LPS increased the expression of M1, M3, M5 subtype mAChR mRNA, and α4, β2 subunit nAChR. In U937 cells, LPS increased COX-2 mRNA expression. However, activation of mAChR or nAChR alone did not affect COX-2 mRNA expression. nAChR activation suppressed the increase in COX-2 mRNA expression by LPS, whereas mAChR activation did not affect the increase in COX-2 mRNA expression by LPS. From the above results, it was clarified that the expression of mAChR and nAChR increases by Mφ activation. Furthermore, in the COX-2 expression control mechanism in U937 cells, it was suggested that the increase in COX-2 expression due to Mφ activation via TLR4 is suppressed by the mechanism via nAChR.","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15020/00001836","subitem_identifier_reg_type":"JaLC"}]},"item_10002_link_30":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_link_text":"同志社女子大学研究者データベース - 間下　雅士","subitem_link_url":"https://research-db.dwc.doshisha.ac.jp/rd/html/japanese/researchersHtml/3633/3633_Researcher.html"},{"subitem_link_text":"同志社女子大学研究者データベース - 藤井　健志","subitem_link_url":"https://research-db.dwc.doshisha.ac.jp/rd/html/japanese/researchersHtml/2703/2703_Researcher.html"}]},"item_10002_publisher_32":{"attribute_name":"出版地","attribute_value_mlt":[{"subitem_publisher":"京田辺"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"同志社女子大学学術情報部"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN0016561X","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04180038","subitem_source_identifier_type":"ISSN"}]},"item_10002_text_27":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"同志社女子大学・薬学部・医療薬学科・特任助教"},{"subitem_text_value":"同志社女子大学・薬学部・医療薬学科・6年次生"},{"subitem_text_value":"同志社女子大学・薬学部・医療薬学科・教授"}]},"item_10002_text_28":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Doshisha Women’s College of Liberal Arts, Assistant Professor"},{"subitem_text_value":"Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Doshisha Women’s College of Liberal Arts, 6th Grader"},{"subitem_text_value":"Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Doshisha Women’s College of Liberal Arts, Professor"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"間下, 雅士"},{"creatorName":"マシモ, マサト","creatorNameLang":"ja-Kana"},{"creatorName":"MASHIMO, Masato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5960","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000387478518","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000387478518"},{"nameIdentifier":"30738886","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000030738886/"}]},{"creatorNames":[{"creatorName":"寺尾, 美帆子"},{"creatorName":"テラオ, ミホコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"6346","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤井, 健志"},{"creatorName":"フジイ, タケシ","creatorNameLang":"ja-Kana"},{"creatorName":"FUJII , Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5964","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000080255380","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000080255380"},{"nameIdentifier":"80255380","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000080255380/"}]},{"creatorNames":[{"creatorName":"TERAO, Mihoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6347","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-09-01"}],"displaytype":"detail","filename":"AN0016561X-20200818-13.pdf","filesize":[{"value":"508.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ヒトマクロファージ様細胞株U937細胞におけるアセチルコリン受容体の生理的役割の検討","url":"https://dwcla.repo.nii.ac.jp/record/1887/files/AN0016561X-20200818-13.pdf"},"version_id":"9f1d7a00-9747-45a3-a554-b06bdcc3dccb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Acetylcholine, Cyclooxygenase-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Muscarinic receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nicotinic receptor, U937 cell lne","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヒトマクロファージ様細胞株U937細胞におけるアセチルコリン受容体の生理的役割の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヒトマクロファージ様細胞株U937細胞におけるアセチルコリン受容体の生理的役割の検討"},{"subitem_title":"Roles of acetylcholine receptors in regulation of immune function in human monocytic cell line U937 Cells","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"19","path":["21","345"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-09-01"},"publish_date":"2020-09-01","publish_status":"0","recid":"1887","relation_version_is_last":true,"title":["ヒトマクロファージ様細胞株U937細胞におけるアセチルコリン受容体の生理的役割の検討"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T14:36:28.678416+00:00"}