{"created":"2023-05-15T14:06:32.594155+00:00","id":1747,"links":{},"metadata":{"_buckets":{"deposit":"4dc141fb-77a4-4294-972a-38be84d853c3"},"_deposit":{"created_by":19,"id":"1747","owners":[19],"pid":{"revision_id":0,"type":"depid","value":"1747"},"status":"published"},"_oai":{"id":"oai:dwcla.repo.nii.ac.jp:00001747","sets":["185:336","20:21"]},"author_link":["5981","5980","5982","5984","5960","5983"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-12-26","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"71","bibliographicPageStart":"63","bibliographicVolumeNumber":"69","bibliographic_titles":[{"bibliographic_title":"同志社女子大學學術研究年報"},{"bibliographic_title":"Doshisha Women's College of Liberal Arts annual reports of studies","bibliographic_titleLang":"en"}]}]},"item_10002_description_19":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10002_description_35":{"attribute_name":"ID","attribute_value_mlt":[{"subitem_description":"AN0016561X-20200316-63","subitem_description_type":"Other"}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"T細胞にはムスカリン性およびニコチン性アセチルコリン受容体（mAChRおよびnAChR）が発現している。T細胞のAChRは、細胞傷害性の増強、細胞分裂の促進、細胞内cGMP濃度の上昇などの生理的役割を担っている。本研究では、T細胞の活性化がAChRの遺伝子発現に及ぼす影響および発現調節メカニズムを検討した。マウス脾臓細胞あるいは脾臓細胞から単離したCD4陽性T細胞において、2 日間の抗CD3/CD28抗体刺激は、M1-M5 mAChR mRNAおよびα4、α7、β2 nAChR mRNAの発現を減少させた。この減少作用には、nuclear factor-κBが関与していることが明らかとなった。さらに、2 日間の抗CD3/CD28抗体刺激は、mAChRあるいはnAChRの活性化による細胞内Ca2＋濃度上昇を抑制した。以上の結果より、T細胞の活性化は受容体発現を減少させることによりAChに対する反応性を低下させることが明らかとなった。すなわち、T細胞の活性化は、mAChRおよびnAChRを介した細胞障害性の増強や細胞分裂促進などの生理作用を低下\nさせる可能性が示唆された。\n\nMuscarinic and nicotinic acetylcholine receptors（mAChR and nAChR）are expressed in immune cells including T cells. AChR of T cells plays physiological roles such as the enhancement of cytotoxicity, the promotion of cell division, the elevation of intracellular cGMP concentration. In this study, the effect of T cell activation on the expression of AChR and the mechanism of its expression were examined. In CD4-positive T cells or CD4-negative cells isolated from mouse spleen cells, anti-CD3/CD28 antibody stimulation for 2 days reduced the expression of M1-M5 mAChR mRNAs and α4, α7, β 2 nAChR mRNAs. It was revealed that nuclear factor-κB is involved in this decreasing action. Furthermore,\nstimulation of intracellular Ca2＋ concentration by activation of mAChR or nAChR was suppressed by stimulation with anti-CD3/CD28 antibody for 2 days. From the above results, it was revealed that activation of T cells reduces the reactivity to ACh by decreasing AChR expression. That is, it was suggested that differentiation of T cells may decrease physiological effects such as enhancement of cytotoxicity and promotion of cell division via mAChR and nAChR.","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"論文","subitem_description_type":"Other"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15020/00001706","subitem_identifier_reg_type":"JaLC"}]},"item_10002_link_30":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_link_text":"同志社女子大学研究者データベース - 間下雅士","subitem_link_url":"https://research-db.dwc.doshisha.ac.jp/rd/html/japanese/researchersHtml/3633/3633_Researcher.html"},{"subitem_link_text":"同志社女子大学研究者データベース - 藤井健志","subitem_link_url":"https://research-db.dwc.doshisha.ac.jp/rd/html/japanese/researchersHtml/2703/2703_Researcher.html"}]},"item_10002_publisher_32":{"attribute_name":"出版地","attribute_value_mlt":[{"subitem_publisher":"京田辺"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"同志社女子大学学術情報部"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN0016561X","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"04180038","subitem_source_identifier_type":"ISSN"}]},"item_10002_text_27":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"同志社女子大学薬学部医療薬学科"},{"subitem_text_value":"同志社女子大学薬学部医療薬学科薬理学研究室6年次生"},{"subitem_text_value":"同志社女子大学薬学部医療薬学科薬理学研究室2015年度卒業生"},{"subitem_text_value":"同志社女子大学薬学部医療薬学科薬理学研究室"}]},"item_10002_text_28":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Department of Pharmacology, Faculty of Pharmaceutical Sciences, Doshisha Women’s College of Liberal Arts"},{"subitem_text_value":"Department of Pharmacology, Faculty of Pharmaceutical Sciences, Doshisha Women’s College of Liberal Arts,Undergraduate of 6th grader"},{"subitem_text_value":"Department of Pharmacology, Faculty of Pharmaceutical Sciences, Doshisha Women’s College of Liberal Arts,Graduate of 2015"},{"subitem_text_value":"Department of Pharmacology, Faculty of Pharmaceutical Sciences, Doshisha Women’s College of Liberal Arts"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"間下, 雅士"},{"creatorName":"マシモ, マサシ","creatorNameLang":"ja-Kana"},{"creatorName":"Mashimo, Masato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5960","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000387478518","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000387478518"},{"nameIdentifier":"30738886","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000030738886/"}]},{"creatorNames":[{"creatorName":"坂口, 美咲"}],"nameIdentifiers":[{"nameIdentifier":"5980","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中, 菜穂子"}],"nameIdentifiers":[{"nameIdentifier":"5981","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤井, 健志"},{"creatorName":"フジイ, タケシ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Takeshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5982","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1000080255380","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/1000080255380"},{"nameIdentifier":"80255380 ","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000080255380 /"}]},{"creatorNames":[{"creatorName":"Sakaguchi, Misaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5983","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Nahoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5984","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-03-17"}],"displaytype":"detail","filename":"AN0016561X-20200316-63.pdf","filesize":[{"value":"953.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"T細胞活性化によるアセチルコリン受容体の発現抑制機構の検討","url":"https://dwcla.repo.nii.ac.jp/record/1747/files/AN0016561X-20200316-63.pdf"},"version_id":"8971f010-3bc9-4724-8940-17bd45bfb2ba"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"T細胞活性化によるアセチルコリン受容体の発現抑制機構の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"T細胞活性化によるアセチルコリン受容体の発現抑制機構の検討"},{"subitem_title":"Activation of T cell reduces acetylcholine receptors on immune cells.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"19","path":["21","336"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-03-17"},"publish_date":"2020-03-17","publish_status":"0","recid":"1747","relation_version_is_last":true,"title":["T細胞活性化によるアセチルコリン受容体の発現抑制機構の検討"],"weko_creator_id":"19","weko_shared_id":-1},"updated":"2023-05-15T14:41:36.854601+00:00"}